Workflow Type: Galaxy

Importing single-end multiplexed data (not demultiplexed yet)

Inputs

ID Name Description Type
Barcodes #main/Barcodes Barcode sequences
  • File
Metadata #main/Metadata A tab separated metadata file
  • File
Metadata parameter #main/Metadata parameter Column name of the metadata to be used for demultiplexing
  • string
Reverse complement barcodes #main/Reverse complement barcodes If reverse complement of barcodes is necessary for the analysis, choose Yes.
  • boolean
Sequences #main/Sequences Single-end sequences
  • File

Steps

ID Name Description
5 Input files qiime2 tools input This step allows the input of the files to be analysed and then generate a qza output, necessary for the QIIME2 pipeline. toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2024.10.0+dist.h3d8a7e27
6 Metadata as artifact Import the metadata as artifact toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2024.10.0+dist.h3d8a7e27
7 Demultiplex single-end data qiime2 demux emp-single Demultiplex sequence data generated with the EMP protocol. toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__demux__emp_single/qiime2__demux__emp_single/2024.10.0+q2galaxy.2024.10.0
8 Summarize demultiplexed output qiime2 demux summarize Summarize counts per sample. (Galaxy Version 2023.5.0+q2galaxy.2023.5.0.2) toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__demux__summarize/qiime2__demux__summarize/2024.10.0+q2galaxy.2024.10.0

Outputs

ID Name Description Type
Demultiplexed single-end data #main/Demultiplexed single-end data n/a
  • File
Summary of the demultiplexing #main/Summary of the demultiplexing n/a
  • File

Version History

v0.3 (latest) Created 27th Nov 2024 at 03:02 by WorkflowHub Bot

Updated to v0.3


Frozen v0.3 036407d

v0.1 Created 27th Nov 2024 at 03:02 by WorkflowHub Bot

Updated to v0.1


Frozen v0.1 9dc83f3

v0.2 (earliest) Created 30th May 2024 at 11:35 by WorkflowHub Bot

Updated to v0.2


Frozen v0.2 792997b
help Creators and Submitter
Creator
  • Debjyoti Ghosh
Additional credit

Helmholtz-Zentrum für Umweltforschung - UFZ

Submitter
License
Activity

Views: 1610   Downloads: 287   Runs: 0

Created: 30th May 2024 at 11:35

help Tags

This item has not yet been tagged.

help Attributions

None

Total size: 83.4 KB
Powered by
(v.1.16.0-main)
Copyright © 2008 - 2024 The University of Manchester and HITS gGmbH