Workflows
What is a Workflow?Filters
Basic processing of a QC-filtered Anndata Object. UMAP, clustering e.t.c
Take an anndata file, and perform basic QC with scanpy. Produces a filtered AnnData object.
Takes fastqs and reference data, to produce a single cell counts matrix into and save in annData format - adding a column called sample with the sample name.
Loads a single cell counts matrix into an annData format - adding a column called sample with the sample name. (Input format - matrix.mtx, features.tsv and barcodes.tsv)
pod5_by_pore
A Snakemake workflow to take the POD5 files produced by an Oxford Nanopore sequencing run and re-batch them by pore (ie. by channel).
This is useful if you want to run duplex basecalling because you can meaningfully run "dorado duplex" on a single (or a subset of) the POD5 files.
Know issues
It is assumed all POD5 input files are from the same sequencing run, but this is not checked.
beacon-omop-worker-workflows
GSC (Genotype Sparse Compression)
Genotype Sparse Compression (GSC) is an advanced tool for lossless compression of VCF files, designed to efficiently store and manage VCF files in a compressed format. It accepts VCF/BCF files as input and utilizes advanced compression techniques to significantly reduce storage requirements while ensuring fast query capabilities. In our study, we successfully compressed the VCF files from the 1000 Genomes Project (1000Gpip3), consisting of 2504 samples and 80 ...
GSC (Genotype Sparse Compression)
Genotype Sparse Compression (GSC) is an advanced tool for lossless compression of VCF files, designed to efficiently store and manage VCF files in a compressed format. It accepts VCF/BCF files as input and utilizes advanced compression techniques to significantly reduce storage requirements while ensuring fast query capabilities. In our study, we successfully compressed the VCF files from the 1000 Genomes Project (1000Gpip3), consisting of 2504 samples and 80 ...
This is the workflow for the biodiversity component of the cultural ecosystems digital twin
Type: Shell Script
Creators: Simon Rolph, Chris Andrews, Will Bolton, Dylan Carbone, Jan Dick
Submitter: Simon Rolph
This is the workflow for the recreation potential component of the cultural ecosystems digital twin
Type: Shell Script
Creators: Chris Andrews, Will Bolton, Simon Rolph, Dylan Carbone, Jan Dick
Submitter: Simon Rolph